The Trypanosoma cruzi DNA Real-Time PCR kit coded TCRUZIDNA.CE is intended for the qualitative detection of Trypanosoma cruzi DNA in human blood samples with a simultaneous control of the extraction/amplification reaction through an Internal Control (IC).
The kit has been adapted for the use on the Real-Time Thermacyclers and ABI 7500 Sequence Detection System® (Software SDS version 1.3.1, Applied Biosystems™*) or MX3000P (Software MxPro version 4.01, Stratagene™***).
NTRODUCTION
The protozoan parasite Trypanosoma cruzi, agent of Chagas disease, infected 28 million people in the Americas. The infection can be acquired mainly by triatomine faeces, blood transfusion, transplacental transmission and by an organ transplanted from an infected donor. This parasitic disease shows a variable clinical course, which ranges from asymptomatic cases, to severe chronic stages characterized by low parasitaemia and cardiac and/or gastrointestinal disorders. If untreated, infection is lifelong. Specific treatment of infection by Trypanosoma cruzi has been performed with the only available drugs: nifurtimox and benznidazole, but the current chemotherapies are unsatisfactory and both drugs have frequently toxic side effects.
RINCIPLE OF THE TEST
Importante note: The TCRUZIDNA.CE kit is performed on Trypanosoma cruzi strain Tulahuen (TcIIe).
The TCRUZIDNA.CE Kit is based on a Real Time chemistry which uses specific Primers and Probes,
Trypanosoma cruzi DNA, recovered from the biological sample under investigation through an extraction step, is amplified using Real Time amplification system. The amplified product is detected using a fluorescent reporter dye probe specific for a Trypanosoma cruzi repeated in 10^4 to 10^5 copies in the parasite genome. Heterologous Internal Control (IC) serves as an Extraction/Amplification control for each individually processed specimen aiming to the identification of reaction inhibitors.
An High Positive control (CTRL-H) and a Low Positive control (CTRL-L) are supplied as controls of the PCR reaction.
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