RNA RETROTRANSCRIPTION KIT
The RNA Retrotrascription kit coded RNART.CE is intended for the reverse transcription to cDNA of highly purified RNA coming from Virus (vRNA) with Random Hexamers.
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NTRODUCTION
Reverse transcription polymerase chain reaction (RT-PCR) is aimed to the generation of many copies of a cDNA from an RNA molecule using the enzyme Reverse Transcriptase.
The resulting cDNA can be used as template of a traditional or real-time PCR.
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RINCIPLE OF THE TEST
Viral RNA, recovered from the biological sample under investigation through an extraction step, is retrotrascribed into cDNA using the reagents supplied in the kit.