The VZV DNA Quantitation (QT) Real-Time PCR kit coded VZVDNAQT.CE is intended for the quantitative detection of Varicella-Zoster virus DNA in human plasma and CSF (cerebrospinal fluid) with a simultaneous control of the extraction/amplification reaction through an Internal Control (IC).
The kit has been adapted for the use on the Real-Time Thermacyclers ABI 7500 Sequence Detection System® (Software SDS version 1.3.1, Applied Biosystems™*), MX3000P® (Software MxPro version 4.01, Stratagene™***) and CFX96 (Software CFX manager version 1.7, Biorad™**).
NTRODUCTION
VZV is a member of the alphaherpesviruses family, related to herpes simplex viruses types 1 and 2. VZV causes chicken pox (varicella) during primary infection and shingles (herpes zoster) following the reactivation of latent virus dormant in the dorsal root ganglia. While VZV infections are usually mild, they sometimes result in severe disease, particularly in immunocompromised patients especially in HIV-infected patients and transplant recipients.
The epidemiology of primary VZV infection varies geographically; in temperate climates, VZV infects >90% of children during the first decade of life, whereas in tropical countries primary infection is often delayed until adulthood.
The VZV genome is a linear double stranded DNA molecule encoding at least 69 unique genes, 3 of which are present in two copies. Large portions of the genome share considerable homology with the genomes of HSV-1 and HSV-2. Many genes are well conserved (have similar structure and function) across the alphaherpesviruses. Virions contain one molecule of linear double stranded DNA.
RINCIPLE OF THE TEST
The VZVDNAQT.CE Kit is based on a Real Time chemistry which uses specific Primers and Probes.
VZV DNA, recovered from the biological sample under investigation through an extraction step, is amplified using Real Time amplification system. The amplified product is detected and quantified, against the standard curve using a fluorescent reporter dye probe specific for a VZV unique genomic sequence.
An Heterologous Internal Control (IC) serves as an amplification control for each individually processed specimen aiming to the identification of reaction inhibitors.
An external standard curve is supplied allowing the determination of the viral load.
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