The HHV6 DNA Quantitation Real-Time PCR kit coded HHV6DNAQT.CE is intended for the quantitative detection of the Herpesvirus 6 DNA in human plasma/blood with a simultaneous control of the amplification/extraction reaction through an Internal Control (IC).
The kit has been adapted for the use on the Real-Time Thermacyclers ABI 7500 Sequence Detection System® (Software SDS version 1.3.1, Applied Biosystems™*) or MX3000P® (Software MxPro version 4.01, Stratagene™***) and CFX96® (Software CFX manager version 1.7, Biorad™**).
NTRODUCTION
Human herpesvirus Type 6 is a beta herpes virus that can exists in two variant, A and B, and infects nearly 90% of the population before two years of age. Furthermore there is evidence of HHV6 infection in approximately 10% of febrile infants less than 90 days old. Primary infections in children often result in fever followed by development of exanthema subitum, also know as roseola infantum.
After primary infection , latency is established in myeloid and bone marrow progenitors and exists for the life time of the host. The virus periodically re-activates from this latent state, with HHV6 DNA detectable in healthy adults. Re-activation in immunocompetent individuals are often asymptomatic but in an immunocompromised clinical status it can lead to serious complications.
RINCIPLE OF THE TEST
The HHV6DNAQT.CE Kit is based on a Real Time chemistry which uses specific Primers and Probes.
HHV6 DNA, recovered from the biological sample under investigation through an extraction step, is amplified using Real Time amplification system. The amplified product is detected and quantified, against the standard curve using a fluorescent reporter dye probe specific for a HHV6 unique genomic sequence.
Heterologous Internal Control (IC) serves as an amplification/extraction control for each individually processed specimen aiming to the identification of reaction inhibitors.
A standard curve is supplied allowing the determination of the viral load.
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